Alterations of 8-iso-prostaglandin F2 alpha levels in heart failure patients with preserved and reduced ejection fraction- a case-control study

Introduction: ‎Heart failure (HF) poses a global health challenge with decreased quality of life. HF can be HF ‎with reduced ejection fraction (HFrEF) or preserved ejection fraction (HFpEF). Oxidative ‎stress is implicated in the pathophysiology of heart failure. The study aimed to find the ‎association of the oxidative biomarker 8-iso-prostaglandin F2 Alpha (8-iso-PGF2α) with the ‎severity of heart failure. ‎ Methods:‎ The case-control study was conducted in the Departments of Cardiology and Biochemistry at ‎Sri Ramachandra Institute of Higher Education and Research, India. The study involved 80 HF ‎patients (HFpEF: n=40, HFrEF: n=40) aged between 30 and 75 years of both genders. ‎Enzyme-Linked Immunosorbent Assay analyzed 8-iso-PGF2α. Institutional ethics committee ‎approval was obtained. Statistical analysis was performed using SPSS software version 16. P ≤ ‎‎0.05 was considered statistically significant.‎ Results:‎ HFpEF was more prevalent in the 46-60 age group, whereas HFrEF was more common in the ‎‎61-75 age group. Among HFpEF and HFrEF, 8-iso-PGF2  levels were 910.07 ± 286.97 and ‎‎1185.02 ± 396.75 (p=0.001). 8-iso-PGF2 showed correlation with NT-proBNP and ‎echocardiography.‎ Conclusions: ‎Elevated 8-iso-PGF2α levels suggested a potential link between oxidative stress and heart ‎failure severity, contributing to our understanding of oxidative stress in heart failure