Microneedle Patch for In Situ Neutrophil Monitoring
Monitoring neutrophil levels plays an essential role in diagnosing infections and various diseases, as well as for managing specific treatments such as chemotherapy and radiotherapy. However, the traditional complete blood count technique is invasive and challenging for quick and frequent monitoring of neutrophil. Herein, a button-like microneedle patch for rapid and convenient detection of neutrophil levels is reported without vascular invasion. This patch consists of a hollow microneedle array with a vacuum chamber for extraction of interstitial fluid, a hydrogel sheet for colorimetric detection of neutrophil level based on neutrophil-specific enzyme (myeloperoxidase), and a mobile phone application for data recording and quantification. The detection ability is validated both in vitro and in vivo in a chemotherapy-induced neutropenic rat model. Moreover, this button patch achieves precise measurement of the neutrophil levels in patients' urine and abdominal drainage fluid. This minimally invasive colorimetric patch can potentiate in situ neutrophil monitoring without blood sampling procedures or in-lab apparatus, which can serve as a substitution for routine blood counts especially for patients in less developed areas.
Authors
- Ziyi Lu (ORCID: https://orcid.org/0000-0002-8209-3188)
- Tao Sheng (ORCID: https://orcid.org/0000-0001-9331-2326)
- Ziqi Gao (ORCID: https://orcid.org/0000-0001-6815-0548)
- Yuqi Zhang (ORCID: https://orcid.org/0000-0003-4616-7722)
- Sheng Zhao (ORCID: https://orcid.org/0009-0000-9806-347X)
- Jiasheng Cao (ORCID: https://orcid.org/0000-0002-4047-8899)
- Mingyu Chen (ORCID: https://orcid.org/0000-0001-5113-754X)
- Zhen Gu
- Ruisi Cai (ORCID: https://orcid.org/0000-0002-8106-4238)
- Sarun Juengpanich (ORCID: https://orcid.org/0000-0002-1449-5564)
- Kangfan Ji
- Yuchao Sun (ORCID: https://orcid.org/0000-0001-8165-3932)
- Win Topatana (ORCID: https://orcid.org/0000-0001-8580-1920)
- Hao Wang (ORCID: https://orcid.org/0000-0003-1204-146X)
- Jiachen Chen (ORCID: https://orcid.org/0000-0002-1941-2326)
- Ruijing Shen (ORCID: https://orcid.org/0000-0002-2641-7838)
- Jiahao Hu (ORCID: https://orcid.org/0000-0001-5835-1012)
- Tian‐Ao Xie (ORCID: https://orcid.org/0000-0003-1738-4840)
- Tianen Chen (ORCID: https://orcid.org/0000-0002-4942-3077)
- Wentao Zhang (ORCID: https://orcid.org/0000-0002-0487-4659)
- Shijie Li (ORCID: https://orcid.org/0000-0002-7583-4523)
- Zhengjie Zhao
- Xinmin Yu
- Xiujun Cai (ORCID: https://orcid.org/0000-0002-3073-6401)
- Yukai Shan
- J. Yu
- Jiahuan You
Institutions
- Sir Run Run Shaw Hospital (CN)
- Jinhua Academy of Agricultural Sciences (CN)
- Second Affiliated Hospital of Zhejiang University (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Advanced Science
- Published
- 2025-05-08
- DOI
- https://doi.org/10.1002/advs.202417801
- Citations
- 6
- Primary Topic
- Advancements in Transdermal Drug Delivery
- Type
- article
- Field-Weighted Citation Impact
- 4.74
Funders
- National Natural Science Foundation of China
- Zhejiang University
- Natural Science Foundation of Zhejiang Province