Nationwide multicentre study of Nanopore long-read sequencing for 16S rRNA-species identification
Nanopore sequencing demonstrated promising potential for bacterial identification in a clinical setting. The results prompt further optimization of the protocol to improve detection of a broader range of species. This multicentre study highlights the feasibility of implementing Nanopore sequencing into clinical microbiological laboratories, for improved national precision diagnostics.
Authors
- René Kaden (ORCID: https://orcid.org/0000-0002-2111-9751)
- Anna Söderlund‐Strand (ORCID: https://orcid.org/0009-0001-8886-4385)
- Anna‐Malin Linde
- Susanne Kerje
- Sara Mernelius (ORCID: https://orcid.org/0000-0001-8317-9517)
- Axel Wester (ORCID: https://orcid.org/0000-0003-3634-6591)
- Erika Tång Hallbäck (ORCID: https://orcid.org/0000-0002-0787-8786)
- Andreas Sjödin (ORCID: https://orcid.org/0000-0001-5350-4219)
- Karolina Gullsby
- Lars Feuk (ORCID: https://orcid.org/0000-0003-2355-2919)
- Sofia Brunet
- Anna Grankvist
- Ida Karlsson (ORCID: https://orcid.org/0000-0002-5598-1200)
- Johanna Keskitalo
- Jenny Welander
- Helena Elding Larsson (ORCID: https://orcid.org/0000-0003-3306-1742)
- Daniel Jaén‐Luchoro (ORCID: https://orcid.org/0000-0002-5988-6227)
- Anna Fagerström (ORCID: https://orcid.org/0000-0002-6276-8811)
- Jens Brandenburg
- Martin Vondracek
- Linda Karlsson (ORCID: https://orcid.org/0000-0003-3317-7550)
- Paula Mölling (ORCID: https://orcid.org/0009-0000-1143-9105)
- Linda Berglind
- Karin Elfving
- Malin Bergman Jungeström
- Hinnerk Eilers
- Frans Wallin
- Christoffer Lindsten
- Lars-Ola Arvidsson
- Mimi Lannefors
- Felicia Rylander
- Miriam Geörg
- Lina Guerra-Blomqvist
- Maria Bergdahl
- Jean-Luc Tison
Institutions
- Linköping University (SE)
- Uppsala University (SE)
- Swedish Defence Research Agency (SE)
- National Food Administration (SE)
- Public Health Agency of Sweden (SE)
- Karolinska University Hospital (SE)
- Örebro University (SE)
- Sundsvall Municipality (SE)
- Science for Life Laboratory (SE)
- Sahlgrenska University Hospital (SE)
- Sunderby sjukhus (SE)
- Falun Hospital (SE)
- University Hospital of Umeå (SE)
- Västmanlands sjukhus Västerås (SE)
- Region Jönköpings län (SE)
- Karlstad Central Hospital (SE)
- Länssjukhuset Sundsvall-Härnösand (SE)
- Länssjukhuset i Kalmar (SE)
- Skåne University Hospital (SE)
- Landstinget i Kalmar län (SE)
- Gävle Hospital (SE)
- Centrallasarettet Växjö (SE)
- Hallands sjukhus Halmstad (SE)
- University of Gothenburg (SE)
Publication Details
- Journal
- European Journal of Clinical Microbiology & Infectious Diseases
- Published
- 2025-05-10
- DOI
- https://doi.org/10.1007/s10096-025-05158-w
- Citations
- 4
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 3.22
Funders
- Oxford Nanopore Technologies
- Karolinska Institutet
- Socialdepartementet
- Göteborgs Universitet
- Science for Life Laboratory
- Sahlgrenska Akademin