Imaging-guided deep tissue in vivo sound printing
Three-dimensional printing offers promise for patient-specific implants and therapies but is often limited by the need for invasive surgical procedures. To address this, we developed an imaging-guided deep tissue in vivo sound printing (DISP) platform. By incorporating cross-linking agent-loaded low-temperature-sensitive liposomes into bioinks, DISP enables precise, rapid, on-demand cross-linking of diverse functional biomaterials using focused ultrasound. Gas vesicle-based ultrasound imaging provides real-time monitoring and allows for customized pattern creation in live animals. We validated DISP by successfully printing near diseased areas in the mouse bladder and deep within rabbit leg muscles in vivo, demonstrating its potential for localized drug delivery and tissue replacement. DISP's ability to print conductive, drug-loaded, cell-laden, and bioadhesive biomaterials demonstrates its versatility for diverse biomedical applications.
Authors
- Gwangmook Kim (ORCID: https://orcid.org/0000-0002-7469-408X)
- Alireza Hassani Najafabadi (ORCID: https://orcid.org/0000-0002-8215-4374)
- Zhiyang Jin (ORCID: https://orcid.org/0000-0002-4411-6991)
- Lihong V. Wang (ORCID: https://orcid.org/0000-0001-9783-4383)
- Paul S. Weiss (ORCID: https://orcid.org/0000-0001-5527-6248)
- Elham Davoodi (ORCID: https://orcid.org/0000-0001-8578-9431)
- Di Wu (ORCID: https://orcid.org/0000-0002-6848-668X)
- Yushun Zeng (ORCID: https://orcid.org/0000-0001-5995-4085)
- Gengxi Lu (ORCID: https://orcid.org/0000-0001-7497-050X)
- Ali Khademhosseini (ORCID: https://orcid.org/0000-0002-2692-1524)
- Xiaotian Ma (ORCID: https://orcid.org/0009-0000-8357-9916)
- Mikhail G. Shapiro (ORCID: https://orcid.org/0000-0002-0291-4215)
- Jee Won Yang (ORCID: https://orcid.org/0000-0002-3257-1823)
- Hossein Montazerian (ORCID: https://orcid.org/0000-0001-6972-2667)
- S. Lee (ORCID: https://orcid.org/0009-0004-7266-8568)
- Shervin S Nia (ORCID: https://orcid.org/0000-0002-2538-0340)
- Jiahong Li (ORCID: https://orcid.org/0000-0001-7938-9589)
- Wei Feng Gao (ORCID: https://orcid.org/0000-0002-8503-4562)
- Qifa Zhou (ORCID: https://orcid.org/0000-0003-1527-3020)
- Tzung K. Hsiai (ORCID: https://orcid.org/0000-0003-1734-0792)
- Ehsan Shirzaei Sani (ORCID: https://orcid.org/0000-0002-4609-1505)
- Lei Li (ORCID: https://orcid.org/0000-0001-6164-2646)
- Jason Williams (ORCID: https://orcid.org/0000-0003-0381-5822)
- Jounghyun Yoo (ORCID: https://orcid.org/0000-0003-4253-2382)
- Behnam Sadri (ORCID: https://orcid.org/0000-0002-1510-2646)
Institutions
- University of Southern California (US)
- California Institute of Technology (US)
- California NanoSystems Institute (US)
- University of California, Los Angeles (US)
- Terasaki Foundation (US)
- Rice University (US)
Publication Details
- Journal
- Science
- Published
- 2025-05-08
- DOI
- https://doi.org/10.1126/science.adt0293
- Citations
- 42
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 28.31