High‐Throughput Fluorescence Screening Enables Globally Consistent Identification of ABA Signaling Modulators

Frequent exposure to environmental stresses can potentially reduce plant yield by up to 60%. The regulation of abscisic acid (ABA) signaling, especially its core receptors, is crucial for the adaptation of plants to environmental stress. However, efficient identification of ABA-signaling modulators remains a challenge. Here, a global high-throughput fluorescence screening (GhitFluors) platform is established to efficiently identify ABA signaling modulators. The GhitFluors platform yields a similar tendency for binding affinity to that determined by classical methods. Diopyridin is effectively identified, a regulator with strong binding affinity to Pyrabactin Resistance 1 (PYR1), and more than 80% inhibitory activity against HAB1, which is equivalent to ABA. Additionally, it can also effectively mitigate the adverse effects of drought on plants. The findings promise the start of a new era for achieving high-efficiency identification ABA signaling modulators.

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Publication Details

Journal
Advanced Science
Published
2025-05-08
DOI
https://doi.org/10.1002/advs.202417212
Citations
1
Primary Topic
Plant Stress Responses and Tolerance
Type
article
Field-Weighted Citation Impact
1.75

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High‐Throughput Fluorescence Screening Enables Globally Consistent Identification of ABA Signaling Modulators

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Advanced Science
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article

High‐Throughput Fluorescence Screening Enables Globally Consistent Identification of ABA Signaling Modulators

Rong‐Jie Pei, Zhi‐You Huang, Zhi‐Zheng Wang, Yangyang Gao, Ge‐Fei Hao, Yu‐Guo Zheng, Xiangyang Y. Li, Huimin Chen, Wei Wang, Jianhong Li, Hong Wu, Ting Wen, Chen Yang, Guang‐Fu Yang
article en
1 citations

Abstract

Frequent exposure to environmental stresses can potentially reduce plant yield by up to 60%. The regulation of abscisic acid (ABA) signaling, especially its core receptors, is crucial for the adaptation of plants to environmental stress. However, efficient identification of ABA-signaling modulators remains a challenge. Here, a global high-throughput fluorescence screening (GhitFluors) platform is established to efficiently identify ABA signaling modulators. The GhitFluors platform yields a similar tendency for binding affinity to that determined by classical methods. Diopyridin is effectively identified, a regulator with strong binding affinity to Pyrabactin Resistance 1 (PYR1), and more than 80% inhibitory activity against HAB1, which is equivalent to ABA. Additionally, it can also effectively mitigate the adverse effects of drought on plants. The findings promise the start of a new era for achieving high-efficiency identification ABA signaling modulators.

Advanced ScienceVol. 12(22)
Guizhou University (CN), Central China Normal University (CN), Minzu Normal University of Xingyi (CN), Hubei University (CN)
Innovative Research Group Project of the National Natural Science Foundation of China, National Natural Science Foundation of China, China Postdoctoral Science Foundation, National Key Research and Development Program of China
Openalex Percentile: Top 15%
Plant Stress Responses and Tolerance
1.75
DOIOpen Access PDFOpenAlex
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