Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study
Our prospective, tissue-agnostic approach highlights the feasibility of ctDNA sequencing by a commonplace and affordable method, offering higher sensitivity to detect CNS infiltration with lymphoma than standard cell-analyzing techniques. We accentuate the benefit of a multi-target NGS approach and adequate CSF sampling to obtain satisfactory diagnostic yield. Less invasive liquid biopsy testing by comprehensive NGS complements standard procedures in the diagnostics and management of CNSL patients, especially when encountering limitations.
Authors
- Andrea Marečková (ORCID: https://orcid.org/0000-0001-9096-9680)
- Andrea Janíková (ORCID: https://orcid.org/0000-0002-2647-7769)
- Veronika Navrkalová (ORCID: https://orcid.org/0000-0003-3020-1578)
- Jakub Paweł Porc (ORCID: https://orcid.org/0009-0005-0269-4370)
- Lenka Radová (ORCID: https://orcid.org/0000-0001-8103-148X)
- Václav Kubeš
- Jana Kotašková (ORCID: https://orcid.org/0000-0003-1672-7346)
- Šárka Pospı́šilová (ORCID: https://orcid.org/0000-0001-7136-2680)
- Viera Hrabčáková
- Tomáš Reigl (ORCID: https://orcid.org/0000-0001-7058-5559)
- Samuel Hricko
Institutions
- Central European Institute of Technology (CZ)
- Masaryk University (CZ)
- Central European Institute of Technology – Masaryk University (CZ)
- University Hospital Brno (CZ)
Publication Details
- Journal
- Biomarker Research
- Published
- 2025-05-09
- DOI
- https://doi.org/10.1186/s40364-025-00777-z
- Citations
- 6
- Primary Topic
- CNS Lymphoma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 8.34
Funders
- European Commission
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Ministerstvo Zdravotnictví Ceské Republiky
- Central European Institute of Technology
- Agentura Pro Zdravotnický Výzkum České Republiky
- Masarykova Univerzita