<i>Chlorella pyrenoidosa</i>-Based Antibiotic Liposomal Gel Promotes Wound Healing by Scavenging Biofilm and Accelerating Regeneration

The biofilm functions as a physical barrier for bacteria, enhancing their resistance to antibiotics and contributing to recurrent infections. Therefore, the scavenging of biofilms has become an important strategy for treating chronic infections. In this study, we demonstrated that <i>Chlorella pyrenoidosa</i> (CP) downregulates the biofilm adhesion genes of <i>Staphylococcus aureus</i> and inhibits biofilm formation. Through the combination of CP with the antibiotic drug berberine hydrochloride (BH) and stabilizers (Poloxamer 188 and Poloxamer 407), we developed a biologically active hydrogel system, which we termed the BHLip@CP gel. Under laser irradiation, the BHLip@CP gel generated reactive oxygen species, which assisted BH to effectively inhibit biofilm formation in <i>S. aureus</i> and reduce the production of virulence factors. In addition, the BHLip@CP gel accelerated wound healing in <i>S. aureus</i> infections by promoting angiogenesis and skin regeneration. This study proposes an innovative strategy to simultaneously eliminate bacterial biofilms and promote wound healing via a synergistic approach that combines chemical and photodynamic processes.