Protocol for sampling multidrug-resistant bacteria using artificial plastic substrates in aquatic ecosystems

Artificial plastic substrates (APSs) consist of four plastic polymers that work as traps for pathogenic bacteria and facilitate their sampling and detection. Here, we present a protocol for sampling antibiotic-resistant pathogenic bacteria in aquatic ecosystems using APSs. We describe steps for selecting plastic polymers, followed by their assembly, submergence in aquatic environments, and collection for analysis. This protocol can also help explore plastic's role as a new route for disease exposure for humans and animals. For complete details on the use and execution of this protocol, please refer to Ferheen et al.¹.