IN-VITRO ANTIMICROBIAL ACTIVITY OF PLUMERIA ALBA AND NELUMBO NUCIFERA FLOWER EXTRACT AGAINST HUMAN PATHOGEN

The present study investigates the phytochemical compositions and antimicrobial efficacy of Plumeria alba and Nelumbo nucifera flower extracts obtained using hot, cold and methanol with and without subsequent distillation. Qualitative phytochemical screening revealed the presence of key bioactive compounds including flavonoids, alkaloids, phenols, tannins, terpenoids, glycosides and saponins with notable variation across extraction techniques and plant species. Methanolic and hot extracts, particularly post-distillation, exhibited pronounced antimicrobial effects against human pathogen, suggesting enhanced concentration of active constituents. The findings highlight the therapeutic potential of P. alba and N. nucifera floral components and underscore the influence of extraction and distillation methaods on bioactive yield and efficacy. This comparative analysis provides a scientific basis for their traditional uses and paves the way for future exploration of their phytopharmaceutical applications. Keywords: Flower extract, Phytochemicals, Antimicrobial activity and Minimal Inhibitory Concentration.