Secondary Metabolites Isolation from Endophytic Fungi Diaporthe Arecae and Colletotrichum Gloeosporioides Isolated from Syzygium Cumini Linn

This study reports some secondary metabolites produced from Diaporthe arecae and Colletotrichum gloeosporioides. These endophytic fungi were isolated from Syzygium cumini Linn plant. The fungal strains were identified based on morphological characteristics and DNA sequence analysis. They were isolated from the plant, followed by its small-scale cultivation and extraction with ethyl acetate. Secondary metabolites from the endophytic fungi were isolated through chromatographic separation and recrystallization. The structures of the isolated secondary metabolites were characterized by 1H-NMR, 13C-NMR, and DEPT-135 spectroscopic data. Column chromatographic treatment of the crude ethyl acetate extract of Diaporthe arecae with solvents of different polarity yielded the compound 1 (6.5 mg) and compound 2 (4.7 mg) respectively. In contrast, the crude extract of Colletotrichum gloeosporioides yielded the compound 3 (2 mg) after treatment with solvents of different polarity. Spectroscopic characterization revealed compound 1 as Ergosterol, compound 2 as 3-(3’-(3”-(3”’-acetoxypropanoyloxy) propanoyloxy) propanoyloxy) propanoic acid and compound 3 as a mixture of ergosterol and 4-methoxy phenol, respectively. The isolation of these compounds implies that endophytic fungi from Syzygium cumini may be a prominent source for the discovery of potential bioactive compounds or lead structures for new drug development.