Ratiometric Red-Emitting Probe with Large Stokes Shift and AIE/ESIPT Characteristics for Acetylcholinesterase Imaging
Overexpression of acetylcholinesterase (AChE) plays a pivotal role in disrupting acetylcholine (ACh) signaling, contributing to the neurodegenerative processes observed in Alzheimer's disease (AD). Detecting AChE activity is crucial for understanding disease progression and developing effective therapeutic strategies. In this study, we present a novel fluorescent probe, DPHP-AChE, which leverages large Stokes shifts (188 nm) and ratiometric fluorescence outputs based on aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) mechanisms. This probe enables the efficient and reliable imaging of AChE activity in living systems, offering enhanced sensitivity and increased resistance to aggregation-caused quenching (ACQ). The large Stokes shift improves the signal-to-noise ratio and facilitates more accurate real-time quantification of AChE expression. We believe that this innovative approach opens up new possibilities for the specific detection of AChE, which can be further extended to the diagnosis and monitoring of AChE-related pathologies.
Authors
- Kang‐Nan Wang (ORCID: https://orcid.org/0000-0002-0835-8803)
- X. Hu (ORCID: https://orcid.org/0000-0002-0086-3127)
- Chunbai Xiang (ORCID: https://orcid.org/0000-0002-1880-2816)
- Ting Jiang (ORCID: https://orcid.org/0000-0001-6930-5193)
- Qihang Ding (ORCID: https://orcid.org/0000-0002-2665-9036)
- Xing Wei Yang (ORCID: https://orcid.org/0009-0000-4822-5127)
- Saijun Mo (ORCID: https://orcid.org/0000-0001-7375-3562)
- Ping Gong (ORCID: https://orcid.org/0000-0002-9652-1657)
- Sufang Shi
- Jia Jia
- Lihua Zhou (ORCID: https://orcid.org/0000-0002-8940-1155)
- Jingjing Xiang (ORCID: https://orcid.org/0000-0001-6276-6501)
Institutions
- Shandong University (CN)
- Shenzhen Polytechnic University (CN)
- Chinese Academy of Sciences (CN)
- Zhengzhou University (CN)
- Laboratoire d’Imagerie Biomédicale (FR)
- Shenzhen Institutes of Advanced Technology (CN)
- Qilu Hospital of Shandong University (CN)
- State Key Laboratory of Crystal Materials
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2025-05-09
- DOI
- https://doi.org/10.1021/acs.analchem.5c00136
- Citations
- 3
- Primary Topic
- Electrochemical Analysis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.55
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Natural Science Foundation of Guangdong Province
- National Key Research and Development Program of China