Complete genome sequence of Enterococcus faecium strain GB_C_05 with potential characteristics applicable as a bacteriocin-producing probiotic feed additive
The whole genome of <italic>Enterococcus faecium</italic> GB_C_05, a strain isolated from Sikhye, a traditional Korean rice beverage, was successfully sequenced and analyzed using Oxford Nanopore Technologies. The complete genome sequence of GB_C_05 contains a circular chromosome with a total length of 2,575,440 base pairs (bp) and a guanine + cytosine (GC) content of 38.2%, along with one circular plasmid, which has a length of 230,283 bp and a GC content of 35.2%. Annotation of the GB_C_05 genome revealed 2,756 protein-coding sequences (CDSs), 70 tRNAs, and 18 rRNAs on the chromosome. Notably, CDSs related to bacteriocin synthesis (Enterocin) and carbohydrate metabolism (α-galactosidase, β-glucosidase and α-L-arabinofuranosidase), as well as genes potentially involved in probiotic-associated functions such as adhesion and colonization, were identified. This comprehensive study presents the complete genome sequence of <italic>Enterococcus faecium</italic> GB_C_05, providing insight into the diverse additives utilized in animal farming to enhance nutritional quality and livestock productivity.
Authors
- Yeongjae Chae
- Hyunok Doo (ORCID: https://orcid.org/0000-0003-4329-4128)
- Gi Beom Keum (ORCID: https://orcid.org/0000-0001-6006-9577)
- Eun Sol Kim (ORCID: https://orcid.org/0000-0001-8801-421X)
- Ju-Hoon Lee (ORCID: https://orcid.org/0000-0003-0405-7621)
- Yejin Choi
- Jinok Kwak (ORCID: https://orcid.org/0000-0003-1217-3569)
- Sheena Kim (ORCID: https://orcid.org/0000-0002-5410-1347)
- Hyeun Bum Kim (ORCID: https://orcid.org/0000-0003-1366-6090)
- Haram Kim (ORCID: https://orcid.org/0009-0002-7504-5249)
- Juyoun Kang (ORCID: https://orcid.org/0000-0002-3974-2832)
Publication Details
- Journal
- Journal of Animal Science and Technology
- Published
- 2025-04-23
- DOI
- https://doi.org/10.5187/jast.2025.e40
- Citations
- 2
- Primary Topic
- Probiotics and Fermented Foods
- Type
- article
- Field-Weighted Citation Impact
- 2.32
Funders
- Ministry of Food and Drug Safety