Analysis of differences in metabolism of pyrimoxsulam resistance in different resistant barley varieties

<bold>Objective</bold> This study aims to explore the important metabolites that may be involved in the metabolism of pyrimoxsulam in hulless barley，as well as clarify the metabolism mechanism of pyrimoxsulam in hulless barley，thus providing a theoretical basis for the scientific use of pyrimoxsulam and the selection and breeding of herbicide-tolerant hulless barley varieties. <bold>Method</bold> The leaves of a tolerant hulless barley variety （Qing 0306）and a sensitive variety （Qing 0160）treated with pyrimoxsulam for 0 d，1 d and 6 d were analyzed by liquid chromatography-mass spectrometry （LC-MS）. <bold>Result</bold> With VIP&gt;1.0 and <italic>P</italic>&lt;0.05 as the threshold，598 differential metabolites were screened out，which were mainly flavonoids，terpenoids compounds，alcohols，phthalides and other substances；KEGG metabolic pathway was enriched and analyzed，which was mainly involved in metabolism of metabolites 2-oxocarboxylic acid metabolism，amino acid biosynthesis as well as flavonoid biosynthesis，among which the biosynthesis of flavonoids is the most significant. <bold>Conclusion</bold> Using metabolomics technology，different varieties of hulless barley leaves were analyzed by metabolomics in different periods（0，1，6 d）after pyrimoxsulam stress，the metabolite changes in sensitive and resistant hulless barley varieties after pyrimoxsulam treatment were clarified.This study provides a basis for the subsequent genetic improvement of hulless barley and the scientific application of pesticides.