EFFECT OF STAPHYLOCOCCAL BACTERIOPHAGE ON BIOFILMS OF STAPHYLOCOCCUS AUREUS STRAINS SENSITIVE AND RESISTANT TO CEFOTAXIME AND AZITHROMYCIN DEPENDING ON THE CHEMICAL COMPOSITION OF THEIR MATRIX

Removal of mature biofilms is the main task of therapy, because biofilms are the basement of the pathogenic process. An influence on staphylococcal biofilms can be done by destruction of extracellular matrix. Phages produce a depolymerase that can destroy the exopolysaccharide matrix of biofilms. This treatment is more effective than standard antibiotic therapy. It was showed that the effect of the drug "Bacteriophage staphylococcal liquid" on biofilms of sensitive to cefotaxime and azithromycin strains, characterized by decreasing of absorbance level of eluted dye on 19.1%. A more significant effect of the bacteriophage was found for biofilms of strains resistant to cefotaxime and azithromycin (33.9% decrease in optical density). Study the composition of biofilms matrix based on the specific destruction of its components. It was found that the main component in the biofilm matrix of the studied strains is exopolysaccharides, and their amount is 12.02% higher than in the antibiotic-resistant strain. Аfter treatment with sodium periodate the decreasing of optical density of eluted dye at 47.00±2.87% for antibiotic sensitive strain and 59.02±2.10% – for resistant strain. The results demonstrate the effectiveness of the phages drugs use to control the growth of biofilms formed by Staphylococcus aureus strains, the matrix of that consisting of a large number of exopolysaccharide.