Analysis of single nucleotide polymorphism using KASP technology for identification of domestic pigs breeds

For the first time in the Republic of Belarus, a large-scale bioinformatic analysis of the genomes of Sus scrofa domesticus species, as well as our own molecular genetic studies, was conducted in order to differentiate Duroc, Landrace, Yorkshire, Belarusian Large White, and Belarusian Meat pig breeds. As a result, a list of polymorphic variants with high differentiating potential was formed. The most informative SNPs for differentiating pig breeds are rs332196135, rs81322965, rs322056535, rs80967182, rs81333725, rs80789418, rs319844693, rs80859281, rs80855833. Based on the statistical analysis of genotypes obtained in silico and using competitive allele-specific PCR technology, high values of accuracy and specificity of the proposed models were determined. Methodological recommendations have been developed for the rapid and accurate differentiation of pig breeds.