Special aspects of structural and functional organization of the genome of Pseudomonas amygdali pv. lachrymans 8: a causative agent of angular leaf spot of cucumber

This article presents the results of the sequencing, molecular genetic and comparative analysis of the genome of the phytopathogenic bacterium Pseudomonas amygdali pv. lachrymans 8. The assembled genome sequence has been deposited in the GenBank database of the US National Center for Biotechnology Information (accession numbers: CP075686–CP075690). The calculation of the average nucleotide identity revealed that the genome sequence of strain 8 exhibits 99.87 and 99.79 % similarity with the genome sequences of bacteria P. amygdali pv. lachrymans 814/98 and P. amygdali pv. lachrymans M301315, respectively. The genome of strain 8 was found to be represented by a circular chromosome of 6,054,652 bp with a GC-pair content of 58.11 % and four circular plasmids: pPAL8-01 (77,748 bp, GC-pair content of 56 %), pPAL8-02 (72,398 bp, GC-pair content of 55 %), pPAL8-03 (49,000 bp, GC-pair content of 54 %) and pPAL8-04 (9,600 bp, GC-pair content of 55 %). It is suggested that the route of dissemination of the studied phytopathogen was either parallel from the Netherlands to the USA and Belarus, or first from the Netherlands to the USA and then to Belarus. A comparison of the nucleotide sequence of the strain 8 with the nucleotide sequences of the bacteria P. amygdali pv. lachrymans M301315, NM002 and YM7902 revealed significant genetic rearrangements and determined the localization of a unique region of 19,773 bp.