Phytochemical Evaluation, HPTLC Fingerprinting Analysis and Antioxidants Activity of Swasakasa Nei (SKN); A Traditional Siddha Herbal Formulation

Background: Swasakasa Nei (SKN) is a traditional Siddha herbal formulation documented in ancient texts, recommended for various health conditions including asthma and eosinophilia. Understanding its phytochemical composition, antioxidant activity, and quality control parameters is crucial for ensuring its efficacy and safety. Methods: This study was conducted for phytochemical evaluation, HPTLC fingerprinting analysis, and antioxidant activity assessment of SKN. Phytochemical analysis was performed using standard methodologies, and HPTLC analysis was conducted to identify the phytochemical constituents. Antioxidant activity was assessed using DPPH and nitric oxide scavenging assays. Results: Phytochemical evaluation revealed the presence of alkaloids, flavonoids, glycosides, coumarin, and phenols in SKN. HPTLC fingerprinting identified distinct peaks corresponding to various phytocomponents, providing insights into its composition. Antioxidant activity assays revealed significant scavenging activity against DPPH and nitric oxide radicals, indicating its potential therapeutic benefits. Conclusion: The study highlights the importance of quality control measures for SKN, including phytochemical analysis, HPTLC fingerprinting, and antioxidant activity assessment. These findings contribute to understanding SKN's therapeutic potential and can guide future research on its efficacy and safety.