Detection and Estimation of Gingerol and its derivatives in Zingiber officinale Rhizome collected from different regions of Eastern India

Ginger, scientifically identified as Zingiber officinale Rosc, is a rhizome belonging to a plant that grows underground; it’s popularly utilized as a spice in the food and beverage sectors. Gingerols have various beneficial effects on health, such as reducing inflammation, antioxidant, preventing cancer, relieving pain, protecting the stomach, strengthening the heart, reducing fever, inhibiting blood vessel growth, preventing blood clotting and lowering blood sugar levels. High-performance liquid chromatography (HPLC) can quickly and easily separate, identify and measure the compounds found in ginger rhizome. The rhizomes of Z. officinale Rosc. were found to contain four gingerols using a fast and reliable HPLC method combined with a diode array detector (HPLC-DAD). The four compounds [6], [8], [10]-gingerol, and [6]-shogaol were separated on an Agilent TC (2) C18 column (250 × 4.6 mm, 5 µm) using a mixture of acetonitrile and HPLC water (40:60) at 40°C. The detection wavelength was set to 272 nm, with a flow rate of 1.0 mL/min. Good linear calibration curves (R² = 1) were used to measure each component. This method was successfully used to quantify the four gingerols in Zingiber officinale Rosc. from various locations in Odisha to evaluate their quality.