Identification of Roseomonas xinghualingensis sp. nov. isolated from hospital air

The genus <i>Roseomonas</i> comprises Gram-negative bacteria widely distributed in the environment and can cause human infections. However, the genomic characteristics and pathogenicity of <i>Roseomonas</i> remain poorly studied. Two new Gram-negative, mesothermic, aerobic and coccoid strains (SXEYE001^T and SXEYE002) were isolated from hospital air. Colonies grown on R2A plates at 28 °C for 3 days were round with neat, raised edges and a pale pink colour. Sequencing of the 16S rRNA gene revealed that the two strains are 98.4% similar to '<i>Roseomonas chloroacetimidivorans</i>' BUT 13^T. The 16S rRNA gene sequence was observed to be 97.4% similar to <i>Roseomonas pecuniae</i> N75^T. Genome-based phylogeny demonstrated that <i>Roseomonas harenae</i> CPCC 101081^T and '<i>R. chloroacetimidivorans</i>' BUT-13^Tspecies are close relatives to strains SXEYE001^T and SXEYE002. The average nucleotide identity between the strains and species of the genus <i>Roseomonas</i> was <95.0%. The G+C contents of strains SXEYE001^T and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. Based on comprehensive genotypic, phenotypic, phylogenetic and phylogenomic analyses, we concluded that strains SXEYE001^T and SXEYE002 were indicative of a novel species within the genus <i>Roseomonas</i>, for which the name <i>Roseomonas xinghualingensis</i> sp. nov. is proposed, with SXEYE001^T (=GDMCC 1.3626^T=JCM 35902^T) as the type strain.