Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification

Plastic pollution presents a global challenge, with polyethylene (PE) being among the most persistent plastics due to its durability and environmental resilience. Long-chain alkane (lcAlk) degrading microbes are a potential source of PE-degrading enzymes, as both lcAlk and PE are large hydrophobic compounds that consist exclusively of C-C and C-H bonds. In this work, we employed a multi-omics approach to study the ability of Aspergillus parasiticus MM36, an isolate derived from Tenebrio molitor intestines, to metabolize lcAlk and secrete enzymes that are potentially capable of modifying PE. The fungus was grown with hexadecane (C16) or a mixture of lcAlk (C24 to C36) as carbon sources and culture supernatants were tested daily for their ability to modify PE. Proteomic analysis identified induced oxidases hypothetically involved in lcAlk and PE functionalization. Key enzymes include multicopper oxidases, peroxidases, an unspecific peroxygenase and FAD-dependent monooxygenases. Surfactant proteins facilitating enzymatic and cellular interaction with hydrophobic substrates, such as one hydrophobin, three hydrophobic surface-binding proteins (HsbA) and one cerato platanin, were present in all secretomes. Transcriptomic analysis comparing lcAlk to C16 cultures highlighted the enrichment of oxidoreductase activities and carboxylic acid metabolism in both lcAlk incubation days, with transmembrane transporters and transferases predominating on day 2 and biosynthetic processes on day 3. In C16 cultures, hydrolytic enzymes, including esterases, were upregulated alongside Baeyer-Villiger monooxygenases, suggesting a shift toward sub-terminal hydroxylation. Integrating transcriptomic and secretomic data, we propose a mechanism for lcAlk assimilation by A. parasiticus MM36, involving extracellular oxyfunctionalization, hydrocarbon uptake via surface-modifying proteins and channeling through membrane transporters for energy consumption and biosynthetic processes. This study provides insights into fungal mechanisms for alkane metabolism and highlights their potential relevance to plastic biotransformation.