Evaluating the integration of olive tree pruning fractionation with ternary eutectic solvent after aqueous pre-extraction or dilute acid pre-treatment

• A maximum OTP delignification of 64.3% was achieved by integrating aqueous pre-extraction with ternary ES treatment. • The use of ternary ES enabled the isolation of lignin fractions with uniform properties, regardless of prior treatment. • Dilute acid pretreatment altered the lignin structure, making it more intricate and less soluble in ES. • Lignin fractions isolated with ES exhibited high levels of aliphatic OH groups (5.7–4.6 mmol∙g –1 lignin). Developing lignocellulosic biomass fractionation approaches that valorize lignin besides the conventional polysaccharide conversion routes have been a growing interest in achieving full biomass cascade processing. Additionally, integrating and designing technologies that use environmentally friendly and recyclable solvents following green chemistry is required. Within this context, the present work aims at investigating the integration of pre-treatments, such as aqueous pre-extraction or dilute acid pre-treatment, with the fractionation of the olive tree pruning biomass (OTP) assisted by ternary eutectic solvent (ES) composed of cholinium chloride, p -toluenesulfonic acid and ethylene glycol (ChCl:pTSA:EG; 1:1:9). The impact of both technological integrations on lignin isolation yield and on lignin physicochemical, thermal and antioxidant properties were the major parameters under evaluation. Overall, the most efficient integration was accomplished with aqueous pre-extraction plus ternary ES treatment. Around 64.3 % and 36.6 % delignification were achieved with ES, when using solid fractions obtained from aqueous pre-extraction and dilute acid pre-treatment, respectively. The lignin isolated after aqueous pre-extraction and ES treatment showed a remarkable aliphatic OH content (5.72 mmol∙g −1 ). Finally, the integration of these processes enabled cellulose enrichment of remaining solid fractions that were accessible to enzymes (80.1–90.3 % hydrolysis yield).