Optimization of <i>Ganoderma lingzhi</i> triterpene extraction method and its hypoglycemic activity

To optimize the continuous reflux extraction process of triterpenes from <i>Ganoderma lingzhi</i> using an orthogonal test and to lay a foundation for its industrial application, an orthogonal experiment was carried out for optimization. The optimal conditions were obtained and verified. The continuous reflux extraction technology for triterpenes from <i>G. lingzhi</i> was optimized. The vanillin-glacial acetic acid method was used to determine the content of triterpenes in <i>G. lingzhi</i>. The optimal process parameters were as follows: an ethanol concentration of 80%, an extraction time of 1.5 hr, and a solid-liquid ratio of 1:26. The average extraction yield was 2.412%, with a relative standard deviation of 1.079%. Taking the inhibition rate of α-glucosidase activity as the index of evaluating hypoglycemic activity, <i>G. lingzhi</i> triterpene has a significant effect on hypoglycemic activity. The optimized triterpene reflux extraction process for <i>G. lingzhi</i> is feasible and stable, providing a scientific reference for the extraction of effective components from <i>G. lingzhi</i> in subsequent stages. The red text indicates deleted content, and the yellow text indicates added content. In order to optimize the continuous reflux extraction process of triterpenes from <i>G. lingzhi</i> through orthogonal testing and to establish a foundation for its industrial application, an orthogonal experiment was conducted for optimization purposes. The optimal conditions were determined and validated. Subsequently, the continuous reflux extraction technique for triterpenes from <i>G. lingzhi</i> was optimized. The vanillin-glacial acetic acid method determined triterpene content. Optimal parameters were: ethanol concentration of 80%, extraction time of 1.5 h, and solid-liquid ratio of 1:26, with an average extraction yield of 2.412% (RSD = 1.079%). Using α-glucosidase activity inhibition rate as an index, <i>G. lingzhi</i> triterpenes showed significant hypoglycemic activity. The optimized extraction process is feasible and stable, offering a scientific reference for subsequent extraction of <i>G. lingzhi</i>'s effective components.