Chitosan nanoparticle‐mediated delivery of <scp>dsRNA</scp> for enhancing <scp>RNAi</scp> efficiency in <i>Locusta migratoria</i>
This study has demonstrated that chitosan-based dsRNA nanomaterials can significantly improve the stability of dsRNA in the midgut fluid, enhance RNAi efficiency, and increase insect mortality. Moreover, chitosan-based dsRNA delivery enhances silencing efficiency by increasing the uptake of dsRNA in the epidermal cells. Our results suggest that the use of chitosan nanomaterial for dsRNA delivery is a feasible strategy for advancing the application of RNAi technology in pest management. © 2025 Society of Chemical Industry.
Authors
- Marian Malak (ORCID: https://orcid.org/0000-0002-3468-0716)
- Zhanghua Zeng (ORCID: https://orcid.org/0000-0002-8102-4770)
- Abeer El Wakil (ORCID: https://orcid.org/0000-0003-2117-0677)
- Jianzhen Zhang (ORCID: https://orcid.org/0000-0003-3400-0044)
- Babar Hussain Chang (ORCID: https://orcid.org/0000-0003-2139-3811)
- Kun Yan Zhu (ORCID: https://orcid.org/0000-0001-9372-8030)
- Linjiang Chai (ORCID: https://orcid.org/0000-0001-9750-6259)
- Jianqin Zhang (ORCID: https://orcid.org/0000-0003-2147-622X)
- Zhangwu Zhao (ORCID: https://orcid.org/0000-0002-3006-7770)
- Bernard Moussian (ORCID: https://orcid.org/0000-0002-2854-9500)
- Shuai Li (ORCID: https://orcid.org/0000-0002-3492-7176)
- Yangyang Liu
Institutions
- Centre National de la Recherche Scientifique (FR)
- Shanxi University (CN)
- Kansas State University (US)
- Université Côte d'Azur (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Chinese Academy of Agricultural Sciences (CN)
- Institute of Environment and Sustainable Development in Agriculture (CN)
- Institut Sophia Agrobiotech (FR)
- Sindh Agriculture University (PK)
- Alexandria University (EG)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-05-08
- DOI
- https://doi.org/10.1002/ps.8880
- Citations
- 13
- Primary Topic
- Insect Resistance and Genetics
- Type
- article
- Field-Weighted Citation Impact
- 10.48
Funders
- National Key Research and Development Program of China