Streamlined Identification of Metallopeptides for Intracellular Catalysis Using Positionally Addressable Combinatorial Libraries
The discovery and development of artificial catalysts to carry out bioorthogonal reactions in living cells is a primary goal at the interface of Chemistry and Biology. Current approaches rely on time-consuming trial-and-error methods. As an alternative, we show that positionally addressable combinatorial libraries (SPOT libraries) provide a significant advantage for the efficient identification of novel catalytic metallopeptides. Using these libraries, we were able to rapidly identify catalytic β-hairpin palladopeptides capable of promoting efficient depropargylation reactions in challenging intracellular environments.
Authors
- Carmen González-González (ORCID: https://orcid.org/0000-0003-4244-0955)
- Soraya Learte‐Aymamí (ORCID: https://orcid.org/0000-0002-1177-7638)
- M. Eugenio Vázquez (ORCID: https://orcid.org/0000-0001-7500-985X)
- José L. Mascareñas (ORCID: https://orcid.org/0000-0002-7789-700X)
- José R. Couceiro (ORCID: https://orcid.org/0000-0002-5742-1071)
- Laura Martínez-Castro (ORCID: https://orcid.org/0000-0003-1765-3174)
- Jean‐Didier Maréchal (ORCID: https://orcid.org/0000-0002-8344-9043)
- Pau Martín-Malpartida (ORCID: https://orcid.org/0000-0001-5867-5535)
- María Jesús Macías (ORCID: https://orcid.org/0000-0002-6915-963X)
- Clara Pose-Insua
Institutions
- Institució Catalana de Recerca i Estudis Avançats (ES)
- Universitat Autònoma de Barcelona (ES)
- Universidade de Santiago de Compostela (ES)
- Center for Research in Molecular Medicine and Chronic Diseases (ES)
- Institute for Research in Biomedicine (ES)
Publication Details
- Journal
- ACS Catalysis
- Published
- 2025-05-08
- DOI
- https://doi.org/10.1021/acscatal.5c00525
- Citations
- 1
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Fundación BBVA
- Institute for Research in Biomedicine
- Ministerio de Ciencia, Innovación y Universidades
- Generalitat de Catalunya
- Xunta de Galicia
- Agència de Gestió d'Ajuts Universitaris i de Recerca