Optimization of MALDI Matrices and Their Preparation for the MALDI‐TOF MS Analysis of Oligonucleotides
The ionic matrix 6-aza-2-thiothymine (ATT) with 1-methylimidazole resulted consistently in a reduced standard deviation and achieved high mass precisions. Additionally, we observed that the S/N ratios and mass precision of 3-hydroxypicolinic acid (3-HPA) varied significantly depending on the solvent composition and the presence of additives.
Authors
- Christoph Haisch (ORCID: https://orcid.org/0000-0002-6344-6750)
- Andreas Wieser (ORCID: https://orcid.org/0000-0002-8703-9228)
- Susanne Dietrich (ORCID: https://orcid.org/0000-0001-8688-8992)
- Anja Dollinger
Institutions
- Fraunhofer Institute for Translational Medicine and Pharmacology (DE)
- German Center for Infection Research (DE)
- Technical University of Munich (DE)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2025-05-07
- DOI
- https://doi.org/10.1002/rcm.10061
- Citations
- 1
- Primary Topic
- Mass Spectrometry Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.76
Funders
- Bayerisches Staatsministerium für Wissenschaft und Kunst