Evaluation Of The Effect Of Gamma Irradiation On The Antimicrobial Compounds Of Pleurotus Ostreatus

Oyster mushroom (Pleurotus ostreatus) has high nutritional value, containing biologically active compounds and therapeutic ingredients. Fruiting bodies of P. ostreatus were irradiated with different irradiation dosages of gamma rays of 0.25, 0.5, 1.0, and 2.0 kGy and then extracted using diethyl ether, ethyl acetate, methyl alcohol, ethyl alcohol, chloroform, and water. The antibacterial properties of the extract were evaluated against a strain of Gram-positive bacteria, Staphylococcus aureus, and Gram-negative bacteria, including Pseudomonas aeruginosa, Klebsiella spp., and Escherichia coli, using the disk diffusion assay. All the tested bacteria were sensitive to diethyl ether extract except P. aeruginosa, which was resistant. However, all tested bacteria were resistant to the other extracts. The most effective diethyl ether extract was selected for examination using gas chromatography-mass spectrometry (GC-MS). GC-MS examination revealed the existence of pharmaceutical substances such as cholest-5-en-3-ol, 1,2-benzenedicarboxylic acid, and oleic acid. GC-MS also displayed the synthesis of new chemical compounds, such as methyl 10,12-pentacosadiynoate, as well as the degradation of some chemical compounds that were detected, such as 9-octadecenoic acid (Z), methyl ester, and (Z)-9-octadecenamide was observed.