Influence of iodine-containing complex on the toxicity of drugs used for the treatment of acute respiratory viral diseases and influenza virus in combined application

Context: One approach to assessing pharmacodynamic drug interactions is the practical application of the Chou-Talalay theory and its combination index theorem. The algorithm for studying and analysing the effects of combinations, according to Chou-Talalay, allows for the calculation of the direction and strength of pharmacodynamic interactions using the combination index (CI) values and to establish the dose reduction index (DRI) for each component of the combination. The algorithm allows for the analysis of combinations of different qualitative and quantitative compositions and calculates the result of interaction at any level of toxicological effect in laboratories engaged in drug development and research. Aims: To evaluate the effect of FS-1 on the toxicity of rimantadine, oseltamivir, and acetylsalicylic acid (ASA) in combined in vitro experiments. Methods: The combined cytotoxicity effect of drugs was evaluated using neutral red on MDCK cells. The type of drug interaction was determined using the principle of median-effect and CI by correlation of doses and effects, the ratio of the value of programmed cell death to the value of actual cell death using the Chou-Talalay method. Results: It was found that the combination FS-1/rimantadine, FS-1/oseltamivir, and FS-1/ASA can significantly reduce their toxic properties. The CI of the combined use of FS-1/rimantadine was CI = 2.018; FS-1/oseltamivir CI = 13.726, and FS-1/ASA CI = 1.518. Conclusions: The use of Chou-Talalay algorithm makes it possible to develop recommendations for the rational use and dosing of drug combinations in clinical practice, as well as to assess the prospects for the development of new combined therapeutic technologies.