Sterile seed germination and seedling cultivation of <i>Idesia polycarpa</i>

The results showed that the optimal time was 10 min for <i>I</i>. <i>polycarpa</i> seed disinfection with 0.1% HgCl₂. The most suitable medium for <i>I</i>. <i>polycarpa</i> seed germination was 1/2 MS medium supplemented with GA₃ (1.0 mg·L^-1) and AC (1.0 g·L^-1), achieving a germination rate of 96.0%. A sucrose concentration of 10.0 g·L^-1 was most beneficial for rooting. When using a single plant growth regulator, indole-3-butyric acid (IBA) had the most significant effect on <i>I</i> . <i>polycarpa</i> root induction. The optimal medium for root development was Murashige and Skoog (MS) medium supplemented with IBA (0.3 mg·L^-1) and α-naphthyl acetic acid (NAA) (0.5 mg·L^-1), resulting in a 100% rooting rate and an average of 22.17 roots. These roots had an average length of 3.4 cm and were abundant and vigorous. Tissue-cultured seedlings were transplanted into transparent plastic cups containing a mixed substrate of organic nutrient soil (BALTIC PEAT), perlite, and vermiculite in a ratio of 2:1:1 (V/V/V). They grew vigorously, with a survival rate as high as 96.67%. The findings of this study can provide technical support for the factory breeding of <i>I</i>. <i>polycarpa</i> seedlings.