<i>In vitro</i> untargeted polar metabolomics data from <i>B. cenocepacia</i> and <i>S. aureus</i> biofilm supernatants

Here, we offer a metabolomics dataset generated via high-performance liquid chromatography and high-resolution mass spectrometry analysis of <i>in vitro</i> biofilm supernatant harvested from the human pathogens <i>Burkholderia cenocepacia</i> and <i>Staphylococcus aureus</i>. A total of 618 polar metabolites were identified across all experimental groups.