Web-based GTalign: bridging speed and accuracy in protein structure analysis

Accurate protein structure alignment is essential for understanding structural and functional relationships. Here, we introduce GTalign-web, a web-based implementation of GTalign, a spatial index-driven protein structure alignment tool, designed for accessibility and high-performance structural searches. Benchmarked against the DALI and Foldseek servers, GTalign-web demonstrates superior accuracy while maintaining rapid search times. Its utility is further highlighted in annotating uncharacterized proteins through searches against UniRef30. GTalign-web provides a useful resource for protein structure analysis and functional annotation and is available at https://bioinformatics.lt/comer/gtalign. This website is free and open to all users, and there is no login requirement.