Screening of Primary and Secondary Metabolites Profile of Different Extracts of Cassia fistula Flowers and its Perspectives on the Antimicrobial and Antidiabetic Potential of Active Extract

This article provides an overview of the pharmacological activity and phytochemical constituents found in Cassia fistula flower extract. Some phytonutrients and antioxidants of plant extract may be compromised due to the relatively high concentration of organic solvents. The main goal of this study was to find out how well an aqueous extract from Cassia fistula flowers fights diabetes and germs in a lab setting. The maceration extract yielded the best results among others. The antimicrobial activity of the extract was evaluated using bacterial and fungal strains, while the anti-diabetic activity was assessed at different concentrations, ranging from 10 to 50 μg/mL. The maximum inhibition of 54.07% was observed at a concentration of 50 μg/mL, compared to the standard acarbose, which showed an inhibition of approximately 62.23%. The IC50 value of the flower extract was 49.3494 μg/mL, while that of the standard acarbose was 42.1726 μg/mL. The presence of phytochemicals in the extract was determined using UV and FTIR spectral studies, which are effective methods for extracting a broad range of chemical constituents. The results show that Cassia fistula flower extract has promising anti-diabetic and antimicrobial activity. It can be concluded that Cassia fistula has high alpha-amylase inhibitory activity, and these extracts have the potential to be effective antimicrobial agents against pathogenic microorganisms.