Exploration of Cellulolytic Microbial Consortium as Biodecomposer of Coffee Fruit Skin Waste

<b>Background and Objective:</b> Coffee fruit skin waste contains high cellulose leading to difficulty in degrading them naturally which finally creates environmental pollution. This research is aimed at finding out the compatible cellulolytic microbe in degrading cellulose content of coffee fruit skin waste. <b>Materials and Methods:</b> Media Carboxymethyl Cellulose (CMC) was used to select cellulolytic fungi. These fungi were tested through a consortium test on media to check out whether the microbial consortium was compatible or not. Furthermore, the ability of microbial consortium in degrading lignocellulose of coffee fruit skin waste was examined. <b>Results:</b> It was found that there were five singular isolates and seven consortium isolates able to degrade cellulose in variative cellulolytic index ranging from 0.43-2.75. In the cellulolytic compatibility test of the seven consortium isolates, there were two compatible consortium isolates with a CCI amount above 1, meanwhile, the consortium test result which was inoculated in PDA media, demonstrated the presence of six consortium isolates of non-synergistic due to the forming of inhibition zone in each isolate. A positive interaction happened between <i>Trichoderma asperellum</i> and <i>Aspergillus japonicus</i> (TA/AJ). Likewise, at the cellulolytic degradation capability test, consortium TA/AJ can degrade cellulose faster compared to using one isolate. <b>Conclusion:</b> The consortium of TA/AJ was the best and proved to be compatible in degrading lignocellulolytic of coffee fruit skin waste.

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Publication Details

Journal
Pakistan Journal of Biological Sciences
Published
2025-05-07
DOI
https://doi.org/10.3923/pjbs.2025.302.307
Primary Topic
Bioeconomy and Sustainability Development
Type
article
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article

Exploration of Cellulolytic Microbial Consortium as Biodecomposer of Coffee Fruit Skin Waste

Eka Wisdawati, Nildayanti Nildayanti, Aksal Mursalat, Islah Fauliah et al.
Pakistan Journal of Biological Sciences
Bioeconomy and Sustainability Development
article

Exploration of Cellulolytic Microbial Consortium as Biodecomposer of Coffee Fruit Skin Waste

Eka Wisdawati, Nildayanti Nildayanti, Aksal Mursalat, Islah Fauliah, Hasmi Aprelia
article en

Abstract

<b>Background and Objective:</b> Coffee fruit skin waste contains high cellulose leading to difficulty in degrading them naturally which finally creates environmental pollution. This research is aimed at finding out the compatible cellulolytic microbe in degrading cellulose content of coffee fruit skin waste. <b>Materials and Methods:</b> Media Carboxymethyl Cellulose (CMC) was used to select cellulolytic fungi. These fungi were tested through a consortium test on media to check out whether the microbial consortium was compatible or not. Furthermore, the ability of microbial consortium in degrading lignocellulose of coffee fruit skin waste was examined. <b>Results:</b> It was found that there were five singular isolates and seven consortium isolates able to degrade cellulose in variative cellulolytic index ranging from 0.43-2.75. In the cellulolytic compatibility test of the seven consortium isolates, there were two compatible consortium isolates with a CCI amount above 1, meanwhile, the consortium test result which was inoculated in PDA media, demonstrated the presence of six consortium isolates of non-synergistic due to the forming of inhibition zone in each isolate. A positive interaction happened between <i>Trichoderma asperellum</i> and <i>Aspergillus japonicus</i> (TA/AJ). Likewise, at the cellulolytic degradation capability test, consortium TA/AJ can degrade cellulose faster compared to using one isolate. <b>Conclusion:</b> The consortium of TA/AJ was the best and proved to be compatible in degrading lignocellulolytic of coffee fruit skin waste.

Pakistan Journal of Biological SciencesVol. 28(5)
Responsible consumption and production
Openalex Percentile: Top 86%
Bioeconomy and Sustainability Development
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Exploration of Cellulolytic Microbial Consortium as Biodecomposer of Coffee Fruit Skin Waste — Eka Wisdawati, Nildayanti Nildayanti, et al. · Pakistan Journal of Biological Sciences (2025) | TGRS Research Map | TGRS