Hypoxia and Hypoxia‐Reoxygenation Potentiate <scp> <i>Helicobacter pylori</i> </scp> Infection and Gastric Epithelial Cell Proliferation
Hypoxia and HIF1 mediated upregulation of CEACAM6 in GECs. CEACAM6 significantly promoted ROS generation by inducing NOX4 in hypoxic GECs. HIF1α, CEACAM6, and NOX4 upregulation was detected in gastritis and GC tissues. H. pylori infection significantly increased in hypoxia-exposed GECs as compared to normoxic GECs. Infection of hypoxia-reoxygenated GECs also resulted in significantly increased CEACAM6 and NOX4-mediated ROS generation compared to normoxic GECs. In addition, adhesion of H. pylori, cytotoxin-associated gene A (CagA) translocation, and GEC proliferation were significantly enhanced in hypoxia-reoxygenated GECs. Collectively, this study established that hypoxia and hypoxia-reoxygenation of GECs facilitate H. pylori infection and infection-mediated GEC proliferation.
Authors
- Pratyush Kumar Padhan (ORCID: https://orcid.org/0000-0003-3551-2172)
- Asima Bhattacharyya (ORCID: https://orcid.org/0000-0001-7292-6644)
- Shivaram Prasad Singh (ORCID: https://orcid.org/0000-0002-8197-2674)
- Indrajit Poirah
- Ashish Kumar Mishra (ORCID: https://orcid.org/0000-0002-1364-330X)
- Pragyesh Dixit (ORCID: https://orcid.org/0000-0001-6907-3767)
- Niranjan Rout (ORCID: https://orcid.org/0000-0002-8501-5994)
- Debashish Chakraborty (ORCID: https://orcid.org/0009-0009-5510-8899)
- Gaurav Nath (ORCID: https://orcid.org/0000-0002-6384-0854)
- Hassan Ashktorab (ORCID: https://orcid.org/0000-0002-4048-4666)
- Supriya Samal
- S.P. Chakraborty (ORCID: https://orcid.org/0000-0001-6459-0175)
- Duane T. Smoot
Institutions
- Meharry Medical College (US)
- Howard University (US)
- National Institute of Science Education and Research (IN)
- Homi Bhabha National Institute (IN)
- Institute of Physical Chemistry (PL)
- Polish Academy of Sciences (PL)
Publication Details
- Journal
- Cancer Medicine
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1002/cam4.70860
- Citations
- 3
- Primary Topic
- Helicobacter pylori-related gastroenterology studies
- Type
- article
- Field-Weighted Citation Impact
- 6.02
Funders
- Department of Atomic Energy, Government of India