Spatially barcoding single nuclei to enable single-cell spatial transcriptomics

Single-cell sequencing and spatially-resolved sequencing are two complementary techniques that allow researchers to explore how tissues are constructed and altered during aging and disease. However, rather than performing two experiments, it is often practically, financially, and technically preferable to be able to directly map single-cell sequencing profiles directly back to their location within a tissue to understand how and why the cell has acquired its phenotype. This article details how a novel technology called Slide-tags enables single-cell sequencing whilst preserving the spatial address of profiled cells.