The potential of biogas production and effects of alfalfa silage under the synergistic influence of <i>Lactobacillus acidophilus</i> and <i>Rosa roxburghii</i> pomace waste on the fermentation quality and bacterial community

Considering the increasing global energy demand and urgent environmental issues, exploring prospective resources for bioenergy production is imperative. However, the biomass of legume perennials may serve as an inexpensive and stable source of clean energy for modern society due to its wide availability and broad range of sources. In addition, the combination of RP and <i>Lactobacillus acidophilus</i> application increased the abundance of Lactobacillus, inhibited the growth of Kosakonia, and promoted anaerobic fermentation, which had beneficial synergistic effects on biomass retention and biogas production in alfalfa samples. Coinoculation improvements with RP and <i>Lactobacillus acidophilus</i> observed here are expected to reduce costs associated with CH₄ conversion bioprocesses and increase CH₄ production.