Generation of an anti-CD5 CAR knock-in human induced pluripotent stem cell line using CRISPR/Cas9 technology

Theanti-CD5 chimeric antigen receptor (CAR)isa genetically engineered immune cell therapydevelopedto targetCD5-associatedhematologic malignancies,such asT-cell lymphoma and leukemia.UsingCRISPR/Cas9-mediated gene targeting, wegeneratedananti-CD5 CAR knock-in human induced pluripotent stem cell (iPSC) linethat stably expresses the CAR constructand is detectable via FLAG tag and GFP markers. Thisengineeredcell linemaintainsstem cell morphology,displaysa normal karyotype, andexhibits robustexpression ofpluripotency markers while retaining differentiation potential.

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Publication Details

Journal
Stem Cell Research
Published
2025-05-06
DOI
https://doi.org/10.1016/j.scr.2025.103731
Citations
1
Primary Topic
CRISPR and Genetic Engineering
Type
article
Field-Weighted Citation Impact
0.81

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article

Generation of an anti-CD5 CAR knock-in human induced pluripotent stem cell line using CRISPR/Cas9 technology

Han Yan, Shuoting Wang, Jiajun Liu, Xinrui Guo et al.
1 citations
Stem Cell Research
CRISPR and Genetic Engineering
0.81
article

Generation of an anti-CD5 CAR knock-in human induced pluripotent stem cell line using CRISPR/Cas9 technology

Han Yan, Shuoting Wang, Jiajun Liu, Xinrui Guo, Tiancheng Zhou
article en
1 citations

Abstract

Theanti-CD5 chimeric antigen receptor (CAR)isa genetically engineered immune cell therapydevelopedto targetCD5-associatedhematologic malignancies,such asT-cell lymphoma and leukemia.UsingCRISPR/Cas9-mediated gene targeting, wegeneratedananti-CD5 CAR knock-in human induced pluripotent stem cell (iPSC) linethat stably expresses the CAR constructand is detectable via FLAG tag and GFP markers. Thisengineeredcell linemaintainsstem cell morphology,displaysa normal karyotype, andexhibits robustexpression ofpluripotency markers while retaining differentiation potential.

Stem Cell ResearchVol. 86
Sun Yat-sen University (CN), Guangzhou Institutes of Biomedicine and Health (CN), Third Affiliated Hospital of Sun Yat-sen University (CN)
National Natural Science Foundation of China
Zero hunger
Openalex Percentile: Top 28%
CRISPR and Genetic Engineering
0.81
DOIOpen Access PDFOpenAlex
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Generation of an anti-CD5 CAR knock-in human induced pluripotent stem cell line using CRISPR/Cas9 technology — Han Yan, Shuoting Wang, et al. · Stem Cell Research (2025) | TGRS Research Map | TGRS