Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli
Our results affirm the viability of the previously developed auto-induction strategy also for a functional hydrogenase with the complex maturation process. This significantly accelerates the process and enhances hydrogenase productivity.
Authors
- Peter B. Neubauer (ORCID: https://orcid.org/0000-0002-1214-9713)
- Qin Fan (ORCID: https://orcid.org/0000-0001-5801-8690)
- Matthias Gimpel (ORCID: https://orcid.org/0000-0002-5136-8169)
- Francisco de la Fuente-Kratzborn
Institutions
- Technische Universität Berlin (DE)
Publication Details
- Journal
- Biotechnology Letters
- Published
- 2025-05-05
- DOI
- https://doi.org/10.1007/s10529-025-03594-4
- Citations
- 1
- Primary Topic
- Metalloenzymes and iron-sulfur proteins
- Type
- article
- Field-Weighted Citation Impact
- 0.36
Funders
- Deutsche Forschungsgemeinschaft
- Einstein Stiftung Berlin
- Technische Universität Berlin