An ecofriendly approach for removal of oil contaminated soil: A biosurfactant study

<p>Oil sludge which is entrapped within soil where its spills. It causes serious problems to the environment as well as both animal and human who interact with them. The hydrocarbons in the sludge penetrate from the top soil into the subsoil slowly, presenting a direct risk of contamination to subsoil and groundwater. On the other hand, the light hydrocarbons in the oil sludge vaporize, leaving behind a layer of oil containing dust of soil which blows upwards to pollute the air. Therefore, the oil sludge should be treated to prevent harm to environment. In this present study, oil contaminated soil was taken for the research work. The soil samples were isolated and their growth characteristics, DNA isolation and DNA estimation was done by using electrophoresis and diphenylamine method. From the results the growth characteristics and DNA quantification confirms that <em>Bacillus subtilis</em> will act as a good surfactant and ecofriendly microorganism. This study will enhance the growth of biosurfactant (bioremediation) for our sustainable environment.</p> <p>published by the <a href="https://innspub.net/international-journal-of-biosciences/"><strong>International Journal of Biosciences (IJB)</strong></a></p>