Development of a double-antigen sandwich ELISA for rapid and accurate detection of antibodies against Capripoxvirus
In this study, a double-antigen sandwich ELISA was developed based on the 122 protein with high immunogenicity and conservation during early viral replication, aiming to detect antibodies against Capripoxvirus. This method features high sensitivity and specificity, is cost-effective, simple, reproducible, and suitable for extensive testing. It can detect antibodies at an early phase and serves as a powerful tool for epidemic monitoring, prevention, and control.
Authors
- Juncong Luo
- Hong Tian (ORCID: https://orcid.org/0000-0001-7991-8559)
- Huancheng Liao
- Zhengwang Shi
- Fan Zhang (ORCID: https://orcid.org/0000-0003-4275-4786)
- Lu Feng
- Xintai Shi
- Haixue Zheng (ORCID: https://orcid.org/0000-0001-6850-1379)
- Tao Xi
- Jie Chen
- Wanying Wang (ORCID: https://orcid.org/0009-0006-2300-3601)
- Yongyu Lin
- Yuqian Zhu
Institutions
- Lanzhou Veterinary Research Institute (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Lanzhou University (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-05-05
- DOI
- https://doi.org/10.1128/spectrum.02729-24
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00