Development of a second harmonic generation microscope optimized for biomaterial studies

Abstract A Second harmonic generation (SHG) microscope optimized for biomaterials was developed. The set-up has a non-collinear counter propagating geometry, that allows for the polarization tuning of incoming and outgoing beams. The developed microscope has a resolution of 1.1 µm/line pair. It makes use of a highly sensitive and high-resolution CCD camera that allows for the imaging of delicate biomaterial samples in short acquisition times without burning. This is the first and only functional SHG microscope set-up in the Philippines.