Multiplex real-time reverse transcription recombinase-aided amplification assay for the detection of SARS-CoV-2, influenza A virus, and respiratory syncytial virus

It is well established that SARS-CoV-2, IAV, and RSV are significant pathogens that elicit respiratory symptoms in humans. In this study, we successfully developed a triplex real-time RT-RAA assay for the simultaneous rapid detection of SARS-CoV-2, IAV, and RSV. This assay is distinguished by its high specificity, sensitivity, and user-friendliness. It addresses the limitations associated with the necessity of specialized biological laboratories and the time-consuming, complex procedures that impede rapid diagnosis. This assay holds significant importance for the early diagnosis and epidemiological surveillance of respiratory tract infections, providing comprehensive and timely diagnostic information for clinical practice. Furthermore, this assay serves as a valuable tool for facilitating extensive and large-scale screening of SARS-CoV-2, IAV, and RSV.