Efficacy and Feasibility of Tissue-Clearing Technique and Three-Dimensional Imaging in the Human Gastrointestinal Tissues Using Illuminate Cleared Organs to Identify Target Molecules
The effectiveness of tissue clearing using LUCID on human gastrointestinal specimens was demonstrated, and the LUCID protocol had minimal impact on specimen morphology and staining. LUCID is expected to be a method that enables comprehensive structural analysis of human gastrointestinal mucosa and lesions that may avoid missing microscopic findings that may occur in split-face pathological assessment.
Authors
- Yosuke Tsuji (ORCID: https://orcid.org/0000-0001-9537-4993)
- Satoshi Ono (ORCID: https://orcid.org/0000-0002-9864-348X)
- Mitsuhiro Fujishiro (ORCID: https://orcid.org/0000-0002-4074-1140)
- Daisuke Ohki (ORCID: https://orcid.org/0000-0002-7400-0914)
- Nobutake Yamamichi (ORCID: https://orcid.org/0000-0002-5741-9887)
- Chihiro Takeuchi (ORCID: https://orcid.org/0000-0002-2746-9189)
- Yuko Miura (ORCID: https://orcid.org/0009-0000-7776-5886)
- Hiroya Mizutani
- Hiroshi Onodera
Institutions
- Tokyo University of Technology (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Digestive Diseases
- Published
- 2025-05-05
- DOI
- https://doi.org/10.1159/000546173
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japanese Foundation for Research and Promotion of Endoscopy
- Japan Society for the Promotion of Science