From in vitro development to accessible luminal interface of neonatal bovine-derived intestinal organoids
Optimized methods for adult bovine intestinal organoids and rectal organoid-derived monolayers are applicable to neonatal intestinal epithelial stem cells. Organoids cultured from 14-day-old calves captured key aspects of the multicellularity and functionality of the native epithelium. Future work should focus on adapting monolayer culture methods for additional gut regions, particularly the proximal gastrointestinal tract. Neonatal rectal monolayers represent a promising platform for advancing veterinary research, agricultural innovation, and studies of zoonotic disease.
Authors
- Claire R. Burbick (ORCID: https://orcid.org/0000-0003-4185-7775)
- Minae Kawasaki (ORCID: https://orcid.org/0000-0001-7149-7315)
- Yoko M. Ambrosini (ORCID: https://orcid.org/0000-0002-9543-2660)
- Craig S. McConnel (ORCID: https://orcid.org/0000-0002-8085-5302)
- Gerald D. Dykstra (ORCID: https://orcid.org/0009-0002-2182-0836)
Institutions
- Washington State University (US)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-05-06
- DOI
- https://doi.org/10.1186/s12917-025-04773-1
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Agriculture
- Washington State University
- National Institutes of Health
- National Institute of Food and Agriculture