Protocol for detection of Oropouche viruses from human serum
Oropouche virus (OROV), an arthropod-borne virus, has reemerged in the Americas. In 2023-2024, the largest quantitative reverse-transcription PCR (RT-qPCR)-confirmed outbreak of OROV was reported and was associated with severe clinical outcomes for the first time. Moreover, a dramatic increase in cases is unfolding in early 2025. Here, we provide a protocol for detecting OROV from human serum. We describe steps for viral isolation, titration, and an in-house RT-qPCR validated in the field. We then detail procedures for measurement of anti-OROV-neutralizing antibodies. For complete details on the use and execution of this protocol, please refer to Ciuoderis et al.<sup>1</sup> and Usuga et al.<sup>2</sup>.
Authors
- Lina Yepes (ORCID: https://orcid.org/0000-0003-2813-5989)
- Juan P. Hernández-Ortíz (ORCID: https://orcid.org/0000-0003-0404-9947)
- Laura S. Perez‐Restrepo (ORCID: https://orcid.org/0000-0002-1734-3697)
- Isabel Y. Moreno (ORCID: https://orcid.org/0000-0002-4640-4364)
- Jorge E. Osorio (ORCID: https://orcid.org/0000-0002-7474-6150)
- Cole Knuese (ORCID: https://orcid.org/0000-0003-4274-0923)
- Jaime Úsuga (ORCID: https://orcid.org/0009-0005-2830-908X)
- Daniel Limonta (ORCID: https://orcid.org/0000-0002-5128-6542)
- Vanessa Vargas (ORCID: https://orcid.org/0009-0000-9703-6517)
Institutions
- University of Wisconsin–Madison (US)
- Universidad Nacional de Colombia (CO)
- National Institute of Health (AM)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-05-05
- DOI
- https://doi.org/10.1016/j.xpro.2025.103805
- Citations
- 1
- Primary Topic
- Viral Infections and Vectors
- Type
- article
- Field-Weighted Citation Impact
- 1.40