Genome editing of TXNIP in human pluripotent stem cells for the generation of hepatocyte-like cells and insulin-producing islet-like aggregates
Our study demonstrates that TXNIP deficiency does not improve the differentiation or functionality of HLCs and SC-islets. We present the generation and characterisation of TXNIP<sup>-/-</sup> and TXNIP<sup>+/+</sup> H1-hESCs, HLCs, and SC-islets as valuable models for future studies on the role of TXNIP in metabolic cell biology.
Authors
- Mariana Nunes (ORCID: https://orcid.org/0000-0002-4733-5778)
- Bernat Elvira (ORCID: https://orcid.org/0000-0003-0434-2238)
- Daria Ezeriņa (ORCID: https://orcid.org/0000-0002-3104-7093)
- David Hay (ORCID: https://orcid.org/0000-0002-6056-6394)
- Leonardo Traini
- Esteban N. Gurzov (ORCID: https://orcid.org/0000-0003-4642-0273)
- Valerie Vandenbempt (ORCID: https://orcid.org/0000-0003-3104-8499)
- Joris Messens (ORCID: https://orcid.org/0000-0002-2128-8264)
- Mayank Bansal (ORCID: https://orcid.org/0000-0002-5798-5352)
- C. E. Buss (ORCID: https://orcid.org/0000-0003-2495-3618)
- Javier Negueruela (ORCID: https://orcid.org/0000-0001-7967-5067)
- Israel Pérez-Chávez (ORCID: https://orcid.org/0000-0002-3270-194X)
- Wadsen St‐Pierre‐Wijckmans (ORCID: https://orcid.org/0009-0005-1080-6117)
- Ao Li (ORCID: https://orcid.org/0000-0001-7861-6313)
- Francisco Ribeiro-Costa
Institutions
- Université Libre de Bruxelles (BE)
- Vrije Universiteit Brussel (BE)
- Vlaams Instituut voor Biotechnologie (BE)
- MRC Centre for Regenerative Medicine (GB)
- VIB-VUB Center for Structural Biology (BE)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-05-04
- DOI
- https://doi.org/10.1186/s13287-025-04314-5
- Citations
- 4
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 8.03
Funders
- Eli Lilly and Company
- Juvenile Diabetes Research Foundation United States of America
- European Foundation for the Study of Diabetes
- Fonds De La Recherche Scientifique - FNRS
- Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture
- H2020 European Research Council