Assessment of response surface methodology-optimized polyherbal feminine hygiene wash against candida albicans

Feminine hygiene products are widely used for personal care, yet their antimicrobial properties, particularly against fungal pathogens like Candida albicans, require further exploration. Vaginal infections, particularly those caused by Candida albicans, are a major health concern among women of all age groups. These infections manifest as itching, burning, vaginal pain, swelling, and abnormal discharge, significantly impacting women's quality of life. The use of polyherbal formulations offers a promising alternative to conventional antifungal treatments due to their synergistic therapeutic effects. This study focuses on the optimization of a polyherbal feminine hygiene wash using Response Surface Methodology (RSM) to enhance its antifungal efficacy against Candida albicans. The formulation was prepared using ethyl acetate extracts of Acacia nilotica, Butea monosperma, and Terminalia bellirica in a hydroxypropyl methylcellulose (HPMC) gel base. In the present investigation a comparative analysis was done amongst experimental formulation, V-Wash, Arogita and Ayouthveda. An in vitro analysis was conducted to evaluate the inhibitory effects of these products on the growth and viability of Candida albicans. The formulation was assessed for its physicochemical properties, including texture, pH, buffer capacity, specific gravity, surface tension, solid contents and total phenolic contents, along with in vitro antifungal activity against C. albicans. The results indicate that the poly-herbal vaginal wash exhibit acceptable physical characteristics and maintain stability over the short term. In vitro studies reveal that the formulation demonstrated strong antifungal activity, with a zone of inhibition (ZOI) of 16.61 ± 0.76 mm, surpassing some commercially available products. These findings suggest that the formulated poly-herbal vaginal wash is a promising alternative for the management and prevention of Candida albicans infections, offering a natural and effective solution for improving intimate hygiene.