Thermal Optimization, pH Sensitivity, Growth Dynamics and Bioactivity of Streptomyces Isolates from soil samples in Anyigba, Kogi state

This study investigates the optimal growth conditions of Streptomyces isolates from soil samples collected in Anyigba, Kogi State, Nigeria, focusing on temperature, pH, and incubation period. It also evaluates their antioxidant and cytotoxic potential for industrial and therapeutic applications. The isolates were identified based on morphological and biochemical analyses. Growth optimization showed that most isolates thrived between 30°C and 40°C, while isolate DM 1 demonstrated high-temperature stability at 60°C. Optimal pH ranged from 5.0 to 7.5, with growth peaks occurring between 48 and 120 hours. Antioxidant activity was strongest in isolates YM 3, DMP 3, GA 2, and CSV 2. Cytotoxicity assays revealed that CSV 1 and DMP 3 exhibited the highest potency, with GA 1, GA 2, and YM 4 also showing significant effects at lower concentrations over extended exposure. In contrast, CW isolates displayed minimal cytotoxicity. These findings enhance our understanding of Streptomyces growth optimization and bioactive potential, supporting their application in biotechnology and pharmaceuticals.