Optimization of RfxCas13d Expression in Escherichia coli Host using Response Surface Methodology
This study highlights the significant impact of the four optimized factors on <i>RfxCas13d</i> expression. Under optimized conditions, a soluble protein concentration of 3.6 <i>mg</i>/100 <i>ml</i> cell culture was achieved after purification. It represents the first application of RSM for optimizing <i>RfxCas13d</i> expression, providing a foundation for further refinement of expression conditions. Continued use of RSM in future research will enhance the efficiency of RfxCas13d production for diagnostic and therapeutic applications.
Authors
- Sadegh Khazalpour (ORCID: https://orcid.org/0000-0003-2931-417X)
- Saeid Afshar (ORCID: https://orcid.org/0000-0001-5136-4259)
- Shahin Eghbalsaied (ORCID: https://orcid.org/0000-0002-9030-8679)
- Meysam Soleimani (ORCID: https://orcid.org/0000-0003-2505-8834)
- Sepideh Abbaszadeh (ORCID: https://orcid.org/0000-0003-2280-8687)
Institutions
- Hamedan University of Medical Sciences (IR)
- Bu-Ali Sina University (IR)
Publication Details
- Journal
- Avicenna Journal of Medical Biotechnology
- Published
- 2025-05-04
- DOI
- https://doi.org/10.18502/ajmb.v17i2.18563
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Hamadan University of Medical Sciences