Genome-wide analysis of the BoBZR1 family genes and transcriptome analysis in Brassica oleracea

The BRASSINAZOLE-RESISTANT 1 genes play a crucial role as key regulators in Brassinosteroid (BR) signaling, which affects various plant developmental and stress-responsive aspects. Understanding regulatory mechanisms via BZR1 in modulating target genes has become a main point in research on plant BR signaling networks. Despite this, the BZR1 functioning in B. oleracea is not elucidated. A complete genome-wide analysis identified 12 BZR1 genes in B. oleracea, categorized into three groups based on their gene motif and structural features. These BoBZR1s were found on eight different chromosomes. Synteny analysis between B. oleracea, Arabidopsis, and potato provided perception into their evolutionary characteristics. Promoter regions of BoBZR1 family genes in B. oleracea have shown specific cis-elements associated with hormones, stress, and plant development. The expression analysis toward cuticular wax biosynthesis revealed that BoBZR1-1, BoBZR1-6, BoBZR1-7, and BoBZR1-10 were upregulated in response to cuticular wax biosynthesis. Differential expressions of BoBZR1 genes were observed for all seven different tested tissues. The whole study involved systematic characterization of the BoBZR1 family, and expression patterns, in BR signaling and its extensive involvement in developmental processes in B. oleracea. Results establish a theoretical foundation for deeper investigation of BoBZR1 structure and functions in B. oleracea, specifically toward regulating plant stress.