Evaluating the Effect of Amygdalin on Some Sperm Parameters of Male NMRI Mice

Introduction: Some natural compounds studied in the field of infertility, despite having positive effects, may also have negative effects, including amygdalin, which is abundant in the seeds and kernels of plants of the rose family. The aim of this study was to investigate the effect of amygdalin on some sperm characteristics and testicular structure in mice. Methods: In this experimental study, 40 male NMRI mice were used in five groups. Four groups received concentrations of 6.25 mg/kg, 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of amygdalin, and one group received saline intraperitoneally for 28 days. Blood samples were taken to measure testosterone. In addition, epididymis and testes were used to assess sperm motility and tissue structure, respectively. The data were analyzed using a one-way analysis of variance in GraphPad software. P<0.05 was considered a significant level. Results: The dose of 50 mg/kg body weight of amygdalin significantly increased serum testosterone levels compared to the control group and the dose of 6.25 mg/kg. Further, all doses of amygdalin (6.25 mg/kg, 12.5 mg/kg, 25 mg/kg, and 50 mg/kg body weight) could significantly increase sperm count compared to the control group (P<0.01 and P<0.05). Other characteristics, including body weight, testicular weight, gonadosomatic index, sperm motility, and seminiferous tubule structure, were not affected by amygdalin. Conclusion: The intraperitoneal injection of amygdalin can affect some sperm parameters. This effect is probably due to the improvement of the antioxidant system. However, the effects of amygdalin on the reproductive system probably depend on the injection method, amygdalin dose, and exposure time.